IKAN TRANSGENIK PDF

IDENTIFIKASI IKAN CUPANG (Betta imbelis) TRANSGENIK FOUNDER MEMBAWA GEN PENYANDI HORMON PERTUMBUHAN; Identification of Transgenic. Ikan transgenik F-0 dibuat dengan menggunakan metode transfeksi. Identifikasi dilakukan menggunakan metode RT-PCR. RNA total. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are.

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One of the advances biotechnologies that expected to be a powerful approach for aquaculture development is transgenic technique. This Author published in this journals.

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Data dianalisis menggunakan metode pembandingan nilai cycle threshold. Identification of zygosity in transgenik fish is normally achieved by PCR analysis with genomic DNA template extracted from the tissue of progenies which are derived by mating the transgenic fish and wild-type counterpart. Persilangan antara ikan transgenik tersebut dengan ikan normal menunjukkan transmisi transgen ke keturunannya mengikuti hukum segregasi Mendel.

Pada penelitian ini, kami menggunakan metode PCR real-time kuantitatif krt-PCR untuk menganalisa sigositas pada satu strain ikan zebra Danio rerio transgenik yang membawa gen D6-desaturase-like dari ikan salmon masu, Oncorhynchus masou.

Dengan demikian, sebuah sistem transgenik untuk memodifikasi biosistesa asam lemak pada ikan telah dikembangkan. Salah satu teknik modern yang diduga akan menjadi sarana yang berguna dalam pengembangan akuakultur adalah teknologi transfer gen. Teknik ini dapat berguna dalam program produksi ikan transgenik secara massal dan dalam percobaan dimana faktor sigositas memberikan pengaruh nyata.

Transgen telah ditransmisikan ke ikan generasi F2 dengan mengikuti pola segregasi Mendel. Jurnal Akuakultur Indonesia Publisher: Tingkat ekspresi transgen yang tinggi pada jaringan ikan F2 yang diperiksa telah diperoleh. Thus, we found that the qTR-PCR to be effective for a rapid and precise determination of zygosity in transgenic fish. Jurnal Akuakultur Indonesia Vol 2, No 1 Dengan demikian, metode krt-PCR adalah efektif untuk penentuan sigositas secara cepat dan tepat pada ikan transgenik.

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Mating of those fish with wild-type had revealed transgene transmission to the offspring following expected Mendelian laws. New approaches addressing this problem could be of great help for fish biotechnologists. Jurnal Akuakultur Indonesia Vol 6, No 2 Transgenic individuals were identified by polymerase chain reaction PCR and their expression levels were analyzed by reverse transcription PCR.

This technique could be useful in the establishment of breeding programs for mass transgenic fish production and in experiments in which zygosity effect could have a functional impact. In this experiment, we applied a quantitative real-time PCR qr-PCR method to analyze zygosity in a stable line of transgenic zebrafish Danio rerio carrying masu salmon, Oncorhynchus masou D6-desaturase-like gene.

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Jurnal Akuakultur Indonesia Vol 6, No 1 This review describes various techniques transgeniik gene transfer, persistence and expression of transferred gene, application and future aspect of gene transfer research in aquaculture.

Intensitas dan kapasitas produksi diharapkan meningkat dengan menggunakan pendekatan bioteknologi. Expression of transgene in F2 generation was varied between gransgenik regarding as the mosaics of F0 fish.

Teknik ini telah diaplikasikan pada spesies-spesies yang memiliki nilai ekonomis. The D6 fatty acid desaturase is generally considered to be the rate-limiting factor in HUFA biosynthesis.

Rapid transgeniik for identification of transgenic fish zygosity Jurnal Akuakultur Indonesia Vol 6, No 2 Pendekatan baru untuk mengatasi masalah tersebut akan memberikan manfaat besar kepada peneliti bioteknologi perikanan. Ulasan ini menggambarkan variasi metode transfer gen, persistensi dan ekspressi dari gen yang ditransfer, aplikasi dan prospeknya ke depan dari penelitian transfer gen dalam akuakultur.

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The first F1 and second F2 generation was transgemik by crossing the transgenic founder F0 and F1, respectively, with wild-type fish.

The transmission rate of transgene into F1 generation was 4. This technique has been applied to several commercially valuable species. The intensity and capacity of production is expected to increase using biotechnology approach.

Metode ini memerlukan ikan dalam jumlah yang banyak, dan juga waktu serta tenaga. This method needs relatively large amounts of fish material and is time- and labor-intensive. Aquacultural output will need to be increased several fold in order to meet the rising demands for fish in coming years as the increasing of mankind population. Recently, global food security has become a hot issue by the public in national as well as out of the country. Data were analyzed using the comparative cycle threshold method.

Produksi akuakultur diharapkan dapat ditingkatkan beberapa kali lipat untuk memenuhi kebutuhan pangan berupa ikan dimasa-masa mendatang akibat peningkatan populasi manusia.

Now, a transgenic system to study the modification of fatty acid biosynthesis pathways in fish was established. Highly unsaturated fatty acids HUFAespecially eicosapentaenoic acid EPA and docosahexaenoic acid DHA have long been recognized for its beneficial effect for human health and development.